Jobs could be lost at a JD Sports warehouse on Derby Commercial Park.
It follows the retailer signing a 20-year lease on the 514,000 sq ft distribution centre just three years ago.
Now, the company has entered a period of consultation with staff as it reviews the future of the Derby site.
A JD Group spokesperson told the BBC: “We have entered into a period of consultation with colleagues based at our Derby distribution centre.
“This is in relation to a review of the Derby distribution centre. We are doing all we can to look after those colleagues impacted and recognise this may be an unsettling time for them.”
Elsewhere in Derby, it was recently announced that JD will more than double the size of its existing 9,397 sq ft presence at Derbion. Set to open towards the end of this year, the new 20,175 sq ft flagship store will showcase brands including Nike, Adidas, The North Face, Jordan and EA7.